Rapid identification of bacteria using a multiplex polymerase chain reaction system for acute abdominal infections
نویسندگان
چکیده
Purpose Acute abdominal infections can be fatal if the causative organism (s) are misidentified. The spread of antimicrobial-resistant bacteria has become a serious problem worldwide, making antibiotic selection extremely difficult. Using quantitative metagenomic analysis, we evaluated commercial multiplex polymerase chain reaction (PCR) system (FilmArray™, bioMérieux, Marcy-l’Étoile, France) for rapid identification bacteria. Methods cases 10 patients with acute were enrolled in this retrospective study. There six perforated peritonitis and four intraabdominal abscess. Fluid collected from surgical examined. Results All specimens tested positive microorganisms culture, involved two or more microorganisms. PCR system, nine ten found to involve at least one microorganism. One specimen was not included panel. Nineteen 21 (90.5%) isolated by culture detected system. Microorganisms very small numbers reads (19 reads) detectable. Conclusion This showed high detection rate ascites abscesses. may suitable as an affordable these cases.
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ژورنال
عنوان ژورنال: Frontiers in Microbiology
سال: 2023
ISSN: ['1664-302X']
DOI: https://doi.org/10.3389/fmicb.2023.1220651